Ose Vaaiga AotelegaoAgarose Gel Electrophoresis
Agarose gel electrophoresis o se faʻaaogaina lautele i le molecular biology mo le vavaeeseina o nucleic acids, pei ole DNA ma le RNA, e faʻatatau i lo latou tele. O lenei metotia e faʻaaogaina ai se gel e faia mai le agarose, o se polysaccharide masani e maua mai le limu. O le gel e galue o se molecular sieve, e mafai ai e molelaʻau ona feoaʻi i totonu pe a faʻaogaina se eletise eletise. O lenei faiga tuueseese e fesoasoani i tagata suʻesuʻe e suʻesuʻe ma faʻavasega faʻataʻitaʻiga o le nucleic acid e ala i le matauina o fusi maʻoti na faia e tusa ai ma o latou lapopoa. Agarose gel electrophoresis o se meafaigaluega faʻavae mo galuega e pei o le DNA fragment analysis, suʻesuʻega mama, ma suʻesuʻega mole biology.
E masani lava, o le tane electrophoresis gel agarose e taʻua foi le tane electrophoresis faalava. O faʻaoga sili o le electrophoresis gel agarose e aofia ai:
Su'esu'ega Vaega o DNA:
Vaevaega ma au'ili'iliga o vaega DNA e fa'atatau i le lapopoa, e mana'omia mo galuega e pei o le genotyping ma le DNA fingerprinting.
Iloiloga RNA:
Fa'ai'uga o molela'au RNA, fesoasoani i le su'esu'eina o fa'ata'ita'iga o fa'ata'ita'iga ma le fa'amaoni RNA.
Fa'amaoniga o oloa PCR:
Fa'amaoniaina o oloa fa'a-fa'a'aiina o le polymerase chain reaction (PCR) e fa'amautinoa ai le fa'atuputeleina manuia ma maua sa'o le tele o vaega.
Su'esu'ega DNA Plasmid:
Su'esu'ega o le DNA plasmid mo le pulea lelei, fa'amaonia o su'ega fa'apipi'i, ma fa'ailoaina ole DNA toe tu'ufa'atasia.
Iloiloga mama:
Fuafuaina o le mama o fa'ata'ita'iga nucleic acid e ala i le va'ai i mea e ono afaina ai po'o le mama.
Su'esu'ega Biology Molecular:
Meafaigaluega fa'avae i falesu'esu'e o meaola mole mole mo su'esu'ega 'ese'ese, e aofia ai tapenaga fa'asologa o DNA ma fa'atapula'aina o enzyme digests.
Beijing Liuyi Biotechnology Co., Ltd. (muamua Beijing Liuyi Instrument Factory) na faavaeina i le 1970. O se pisinisi tekinolosi faapitoa i le gaosiga o biochemistry ma molecular biology falesuesue instruments.Liuyi ua faapitoa i le gaosiga o meafaigaluega electrophoresis mo le silia ma le 50 tausaga , ma o loʻo i ai le faʻalagolago ma atoatoa le gaosiga o laina mai le mamanu i le asiasiga, ma fale teu oloa, faʻapea foʻi ma le maketiina lagolago. O oloa autu o Electrophoresis Cell (tank / chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Sei o tatou iloa atili e uiga i lana faʻataʻitaʻiga tasi o le agarose gel electrophoresis-DYCP-31DN.
Folasaga o agarose gel electrophoresistane DYCP-31DN
Fua (LxWxH) | 310×150×120mm |
Tele ole Gel (LxW) | 60×60mm, 60×120mm, 120×60mm, 120×120mm |
Selu | 2+3 vaieli, 6+3 vaieli, 8+18 vaieli, ma le 11+25 vaieli |
Selu Mafiafia | 1.0mm, 1.5mm ma le 2.0mm |
Numera o Fa'ata'ita'iga | 2-100 |
Volume Puipui | 650ml |
mamafa | 1.0kg |
O le DYCP-31DN o le Liuyi's agarose gel electrophoresis tane, faʻatasi ai ma se masini faʻapipiʻi e avanoa mo le lafoina o 1-4 fasi gels. E mafai ona faia ni gel laʻititi se lua e pei o le tele 60x60mm ma le tasi gel lautele 120x60mm poʻo se gel umi 60x120mm i le taimi e tasi. Ole gel pito sili e mafai ona lafo ole lapo'a 120x120cm.
O lemafiafia selu ofoinao1.0mm, 1.5mm ma le 2.0mm. Faatasi ai ma le mamanu o le eletise e mafai ona aveese, e matua faigofie lava ona mama ma tausia. "Bridge design" e fa'atatau i le fa'asaoina o vaifofo pa'u i le faagasologa o le electrophoresis. O le fa'aogaina lelei o le pa'u e taua tele mo le fa'atumauina o fanua eletise mautu ma fa'amautinoa le vavae'ese sa'o o mole.
O isi lumana'i taua:
1) O tapuni ma le tino o tane (tapa paʻu) e manino, faʻapipiʻiina, matagofie, umi, faʻamaufaʻailoga lelei, leai se faʻaleagaina o vailaʻau; vailaʻau-teteʻe, faʻamalosi.
2) Electrodes e faia e le platinum mama (o le mama quotient o le uʻamea mamalu ≥99.95%) o loʻo i ai foliga o le corrosion teteʻe o electroanalysis ma tetee atu i le vevela maualuga, o le galuega o le conduction eletise e matua lelei.
3) Fa'ata'ita'i vaieli e masani ona faigata ona va'ai, o le mea lea o le paʻu uliuli i luga o le fata gel e faigofie ai ona matau ma utaina faʻataʻitaʻiga.
4) E leai se malosi pe a e tatalaina le tapuni mo le saogalemu o le tagata faʻaoga.
E tele fa'ata'ita'iga o le eletise o lo'o ofoina atu mo le DYCP-31DN. Ole electrophoresis sili ona vevela paoa sapalai is DYY-6C. Faʻafeiloaʻi e asiasi i la matou upega tafaʻilagi mo nisi faʻataʻitaʻiga o le eletise.
Mo le vaai faalemafaufau i to iagel agarose maDNA poʻo RNA, Liuyi tuʻuina atu UV transilluminators mo tagata faʻatau.WD-9403C, WD-9403B maWD-9403X e avanoa uma mo tagata e filifili.
E aoga tele le electrophoresis gel Agarose mo galuega e pei o le siakiina o le tele o vaega DNA, iloilo le mama o se DNA faʻataʻitaʻiga, poʻo le vavaeeseina o vaega DNA eseese mo le suʻesuʻeina atili.
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Taimi meli: Ian-11-2024